Site-Specifically Modified Protein Synthesis Services

1. Introduction:

Advance your research with our specialized site-specific protein modification and recombinant engineering services. We deliver precise, customizable protein engineering solutions that enable the introduction of defined chemical modifications at exact amino acid residues—strictly preserving tertiary protein structure, stability, and biological activity.

Powered by advanced recombinant expression systems, enzymatic modification platforms, and bioorthogonal chemistry (including Amber Suppression / Genetic Code Expansion), we specialize in complex protein modifications, site-specific conjugation, fluorescent labelling, PEGylation, and epigenetic mimetics (e.g., site-specific histone methylation, acetylation, and phosphorylation). Our integrated pipeline ensures fully characterized, ultra-pure proteins with exceptional batch-to-batch consistency and reproducibility.

From construct design and expression optimization to precision modification, purification, and mass spectrometry characterization, our team offers end-to-end support tailored for drug discovery, epigenetic research, structural biology, and diagnostic applications.

     2. Service Sub-categories

      ① Recombinant Protein Expression & Mutagenesis

• Expression Systems: coli recombinant systems optimized for high-yield protein and histone variant production.
• Construct Engineering: Site-directed mutagenesis, truncation variants (N/C-terminal), and flexible tag incorporation (His-tag, Thrombin cleavage).

      ② Epigenetic & Histone Protein Engineering

• Post-Translational Modifications (PTMs): Site-specific methylation, acetylation, phosphorylation, and ubiquitination mimetics.
• Histone Variants & Nucleosome Assembly: Recombinant Histone H3, H2A, and H2B constructs designed for chromatin and epigenetic assay development.

      ③ Genetic Code Expansion & Bioorthogonal Chemistry

• Amber Suppression (TAG Codon Insertion): Precision incorporation of non-canonical amino acids (ncAAs) at defined residues.
• Bioorthogonal Conjugation: Click chemistry, site-specific fluorescent/biotin tagging, and PEGylation for molecular probes and therapeutic candidates.

      ④ Characterization & Quality Assurance

• Full QC Panel: Intact Mass Spectrometry (LC-MS) for modification verification, SDS-PAGE purity analysis, and solubility/stability assessment.